Table 1.4.
Mechanism | 2-state model |
3-state model (monomeric intermediate) |
---|---|---|
Equilibrium constants and total protein concentration | ||
Definition of molar fraction fN2 = | 1 - fU | 1 - fI - fU |
Definition of molar fraction fI2 = | — | — |
Definition of molar fraction fI = | — | |
Definition of molar fraction fU = | K2fI | |
Fitting equation | Y = YN2fN2 + YUfU | Y = YN2fN2 + YIfI + YUfU |
Mechanism |
3-state model (dimeric intermediate) |
4-state model |
Equilibrium constants and total protein concentration | ||
Definition of molar fraction fN2 = | 1 - fI2 - fU | 1 - fI2 - fI - fU |
Definition of molar fraction fI2 = | ||
Definition of molar fraction fI = | — | |
Definition of molar fraction fU = | ||
Fitting equation | Y = YN2fN2 + YI2fI2 + YUfU | Y = YN2fN2 + YI2fI2 + YIfI + YUfU |
Notes: Abbreviations used are the same as described for Table 1.3, with the representing addition of the N2 native homodimer and I2 representing the homodimeric intermediate. fN1 and fI2 are the mole fraction of the homodimer and of the dimeric intermediate, respectively, and YN2 and YI2 are the amplitudes of the spectroscopic signal for the specified species.