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Example calculations for 55 μM procaspase-3 protein stock solutions for refolding/unfolding kinetic experiments experiments
| Protein stock solutions |
10 M Urea (mL) |
1 M DTT (μL) |
825 μM Proteina (μL) |
Buffer (mL) |
|---|---|---|---|---|
| Unfolded control (1 mL) | 0.8 | 1 | 66.7 | 0.1323 |
| Native (15 mL) | 0 | 15 | 1000 | 13.985 |
| Native control (1 mL) | 0.073 | 1 | 66.7 | 0.8593 |
| Unfolded (15 mL) | 12 | 15 | 1000 | 1.985 |
Initial protein concentration of 825 μM is diluted to 55 μM for kinetic folding experiments.
