Calibration of the delay between driver and reporter expression. Eye discs are shown; anterior rightwards, dorsal upwards. (A,B) GMR directly driving Gal4 (GMR:G); (C,D) GMR driving the MAP kinase-Gal4/Vp16 fusion (GMR:MG); (E,F) GMR driving the nuclear localized MAP kinase-Gal4/Vp16 fusion (GMR:NMG). Reporters (A,C,E) UAS:lacZ, (B,D,F) UAS:GFP. In all cases, Glass antigen is colocalized with the reporter antigen (β-galactosidase or GFP) as indicated. Leading edges of Glass expression (arrowheads) and of reporter expression (arrows).