Table 5.

Summary of Quantitative Studies Concerning Peptide-Mediated Steric Block Oligonucleotide Uptake

Mode of Delivery	Molecules Per Cell for Maximal Splice Correction
LF2000-mediated transfection	~7,000,000
MPGα -mediated transfection	~70,000,000
nuclear microinjection	~300,000

Transfections were performed with 2.5 µM MPGα or 7 µg/ml LF2000 and 278 nM steric block oligonucleotide, respectively, to achieve maximal splice correction. Quantification was performed after 24 h according to the liquid hybridization protocol ([55] and Laufer et al., manuscript in preparation). Molecules per cell were calculated based on the cell number seeded for transfection. For microinjection experiments, molecules per cell were calculated on the basis of the injection volume.