Figure 2.

Effect of BDNF on intracellular Ca²⁺ ([Ca²⁺]_i) responses of ASM cells to acetylcholine (ACh). Under unstimulated conditions, exposure to 10 nM BDNF (but not lower concentrations) significantly increased baseline [Ca²⁺]_i levels. In the absence of BDNF, ACh produced a concentration-dependent increase in peak [Ca²⁺]_i responses. A 30-minute, 1 nM BDNF exposure had a significant enhancing effect only on [Ca²⁺]_i responses to high agonist concentration. Both peak and amplitude of the [Ca²⁺]_i responses were affected. In comparison, 30 minutes of 10 nM BDNF exposure substantially enhanced peak and amplitude responses at different agonist concentrations. Values are means (±SE). *Significant effect of 1 nM BDNF (compared with 0 nM); ^#significant effect of 10 nM BDNF (compared with 0 nM); P < 0.05.