Figure 8.
Effect of TrkB versus p75NTR siRNA on sarcoplasmic reticulum (SR) Ca2+ release. In ASM cells not exposed to TNF-α (top), [Ca2+]i responses to 5 mM caffeine were determined in the absence of extracellular Ca2+. Under these conditions, 10 nM BDNF enhanced SR Ca2+. TrkB siRNA, but not p75NTR, blunted the enhancing effect of BDNF on the [Ca2+]i response to caffeine. In cells exposed overnight to TNF-α (bottom), [Ca2+]i responses to caffeine were increased (compared with non-exposed controls). Under these conditions, additional exposure to BDNF substantially increased caffeine responses. TrkB siRNA (but not p75NTR siRNA) blunted BDNF effects. *indicates significant siRNA (TrkB or p75NTR) effect compared with BDNF only. #Significant BDNF effect compared with control (no BDNF). Values are means ± SE. Values are means ± SE.