Figure 9.

Effect of TrkB versus p75NTR siRNA on store-operated Ca²⁺ entry (SOCE). In ASM cells exposed to zero extracellular Ca²⁺, sarcoplasmic reticulum Ca²⁺ stores were depleted using cyclopiazonic acid, as described previously (16, 22). Reintroduction of extracellular Ca²⁺ was used to trigger SOCE. Exposure to 10 nM BDNF before extracellular Ca²⁺ enhanced SOCE; in cells transfected with TrkB siRNA, but not p75NTR, this enhancing effect of BDNF on SOCE was absent. In cells exposed overnight to TNF-α (bottom), SOCE was increased. BDNF substantially increased SOCE. TrkB siRNA (but not p75NTR siRNA) blunted the BDNF effect on SOCE in TNF-α–exposed cells. *Significant siRNA (TrkB or p75NTR) effect compared with BDNF only; ^#significant BDNF effect compared with control (no BDNF). Values are means (±SE).