Skip to main content
. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: J Chromatogr A. 2009 Aug 21;1216(41):6881–6889. doi: 10.1016/j.chroma.2009.08.044

Fig.4.

Fig.4

(A) Two-dimensional gel electrophoresis (2-DE) of HEK293 nuclear extract. HEK293 nuclear extract (50 µg) was separated by IEF using a pH 3–10 strip (7 cm) combined with 12% SDS-PAGE gel electrophoresis in the second dimension. The 2-DGE gel is stained by silver nitrate. (B) Two-dimensional southwestern blot analysis (2D-SW) of the CAAT enhancer binding protein (C/EBP) in nuclear extract. A 2-DE gel of HEK293 (50 µg) nuclear extract was transferred to nitrocellulose membrane for SW blot analysis. The proteins interacting with C/EBP cognate DNA are indicated by circles. Position off protein MW standard is shown on the left.