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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: J Chromatogr A. 2009 Aug 21;1216(41):6881–6889. doi: 10.1016/j.chroma.2009.08.044

Fig.5.

Fig.5

Scheme of 2D-EMSA and 3D-EMSA. Nuclear extract is incubated with radiolabeled element DNA to form specific DNA-protein complex and analyzed by EMSA. The complex on the non-denature PAGE gel is cut out, crashed and mixed with 1X Laemmli buffer or Rehydration buffer for further separation by SDS-PAGE (2D-EMSA)or two-dimensional electrophoresis (3D-EMSA).