Table 1.
Responses of Mice to Intranasal Immunizations with Inactivated Influenza A/Texas/91 (H1N1) Vaccine1
| Group2 | Serum GMT Neut Ab (log2) |
NS Mean Antibody3 |
Lung Fluid Mean Ab3 |
Lung Virus Mean Titer4 |
|---|---|---|---|---|
| Mixed adj | <2 | 0.25 | 0.25 | 6.7 |
| Vac + PBS | 2.6 | 0.68 | 0.65 | 6.2 |
| Vac + CTB | 6.0 | 1.15 | 1.18 | 2.6 |
| Vac + MPL | 5.6 | 0.94 | 1.45 | 2.2 |
| Vac + IFN | 5.6 | 1.18 | 1.22 | 2.0 |
| Live virus | 5.8 | 2.05 | 1.76 | <1.8 |
1
10 mice/group, 30 microliter dose; vac – 0.15 microgram on day 1, 2, and 28, 29,with PBS or 2.5 microgram CTB, 3.75 microgram MPL or 1X104 IFN u; serum antibody on day 42, nasal secretions and lung fluid antibody on day 45 on nonchallenged mice; challenge on day 42 with lung virus on day 45
2
adj = adjuvant, vac = vaccine, PBS = phosphate buffered saline, CTB = cholera toxin B, MPL = monophosphoryl lipid A, IFN = interferon
3
Mean optical density in Elisa assay; NS = nasal secretions
4
log10/lung