Table 3.
Oligomerization of homomers (WT-βB1 or its mutants alone) and heteromers (WT-βA3 plus WT-βB1 or its mutants mixed at 1:1 molar ratio)
| Homomers |
Heteromers |
||||||
|---|---|---|---|---|---|---|---|
| Distribution (%) | molar mass (kDa) | approx. # of subunits | polydispersity | Distribution (%) | molar mass (kDa) | polydispersity | |
| WT-βB1 | 54.8 | 59.0 ± 1.8 | 2 | 1.53 ± 0.24 | 54.5 | 20.1 ± 1.1 | 1.80 ± 0.16 |
| 41.8 | 31.2 ± 0.8 | 1 | 1.02 ± 0.09 | 27.6 | 68.1 ± 1.1 | 1.09 ± 0.03 | |
| βB1 (59-252) | 34.8 | 75.5 ± 16.8 | 3 | 1.00 ± 0.32 | 45.0 | 67.9 ± 1.8 | 1.11 ± 0.04 |
| 22.0 | 196.2 ± 19.2 | 8 | 1.93 ± 0.33 | 25.2 | 11.3 ± 2.3 | 2.32 ± 0.94 | |
| βB1 (99-252) | 27.5 | 379.3 ± 14.2 | 20 | 1.19 ± 0.03 | 47.7 | 7.7 ± 0.7 | 1.34 ± 0.16 |
| 25.2 | 80.3 ± 4.8 | 4 | 1.70 ± 0.15 | 33.3 | 80.2 ± 1.6 | 1.03 ± 0.03 | |
| βB1 (144-252) | 76.6 | 11.6 ± 1.2 | 1 | 1.03 ± 0.14 | 46.1 | 22.9 ± 2.7 | 1.98 ± 0.28 |
| 10.9 | 58.9 ± 7.1 | 5 | 1.44 ± 0.34 | 41.7 | 220.9 ± 5.1 | 1.02 ± 0.03 | |
| βB1 (149-252) | 86.6 | 14.0 ± 3.1 | 1 | 2.19 ± 0.89 | 62.4 | 6.1 ± 0.3 | 1.25 ± 0.08 |
| 7.4 | 87.8 ± 26.7 | 6 | 7.67 ± 6.78 | 12.0 | 91.0 ± 2.4 | 1.16 ± 0.05 | |
| βB1 (1-234) | 98.7 | 20.7 ± 2.7 | 1 | 12.46 ± 3.86 | 86.3 | 10.6 ± 1.6 | 1.60 ± 0.34 |
| 1.0 | 90.1 ± 24.5 | 5 | 1.05 ± 0.39 | 7.4 | 68.5 ± 5.2 | 1.33 ± 0.14 | |
| βB1 (1-190) | 43.2 | 12,240.0 ± 730.0 | 520 | 1.01 ± 0.08 | 50.6 | 742.2 ± 97.7 | 1.07 ± 0.22 |
| 32.4 | 692.2 28 ± 394.4 | 30 | 2.40 ± 1.90 | 26.1 | 7,727.0 ± 615.0 | 1.47 ± 0.18 | |
| βB1 (1-148) | 90.7 | 29 9.9 ± 0.9 | ½ | 5.35 ± 1.96 | 60.6 | 5.3 ± 0.4 | 1.96 ± 0.30 |
| 4.1 | 132.0 ± 5.8 | 7 | 1.03 ± 0.06 | 24.4 | 63.2 ± 1.3 | 1.04 ± 0.03 | |
| WT-βA3 | 57.9 | 52.9 ± 0.8 | 2 | 1.08 ± 0.02 | N/A | N/A | N/A |
| 24.4 | 6.6 ± 1.0 | ¼ | 4.88 ± 1.74 | N/A | N/A | N/A | |