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. Author manuscript; available in PMC: 2010 Oct 31.
Published in final edited form as: J Immunol Methods. 2009 Aug 14;350(1-2):22–28. doi: 10.1016/j.jim.2009.08.003

Figure 3. Effect of M7/1 on EMAP II-induced migration and apoptosis.

Figure 3

A) Inhibition of EMAP II-induced monocyte migration. Migration assays were performed using Boyden chambers with PBMC. EMAPII protein (1 μg/ml) in the lower well induced a significant migration compared to control medium (*p<0.01). Pre-treatment with neutralizing antibody M 7/1 (10 μg/ml) but not with control rat IgG inhibited pro-EMAP II (1 μg/ml)-induced migration (p**<0.05). B) Inhibition of EMAP II-induced apoptosis of tumor cells. Human breast carcinoma cells incubated with pro-EMAPII protein (1 μg/ml) demonstrated a significant apoptosis as shown by TUNEL (*p<0.01). Pre-treatment of MCF7 cells with neutralizing antibody M 7/1 (10 μg/ml) but not with control rat IgG inhibited EMAP II (1 μg/ml)-induced apoptosis (**p<0.05). Data shown are from a representative experiment performed in triplicates and repeated independently two additional times with similar results.