Figure 2. M-CSFR, Flt3 and GM-CSFR control the development of LP DCs.

A. Dot plots show the % of CD103⁺CD11b⁻, CD103⁺CD11b⁺ and CD103⁻CD11b⁺ cells among DAPI⁻CD45⁺MHCII^hiCD11c^hi SB LP DCs in M-CSFR^−/−, Flt3^−/− and GM-CSFR^−/− mice (right panels) or control WT littermates (left panels). PP were excised from the SB of Flt3^−/−, GM-CSFR^−/− and control littermates, but not from the SB of M-CSFR^−/− and their MCSFR^+/+ control littermates. B. Bar graphs show the relative change of absolute cell counts among SB LP DC subsets in M-CSFR^−/−, Flt3^−/− and GM-CSFR^−/− mice compared to WT mice. Error bars represent mean +/− SD from 4 (M-CSFR^−/−) to 6 (Flt3^−/− and GM-CSFR^−/−) combined experiments. (*) − 0.05>p>0.005, (**) − p<0.005. C. WT CD45.1⁺ mice were lethally irradiated and reconstituted with a mixture of 1:1 CD45.1⁺ WT and CD45.2⁺ Flt3^−/− or GM-CSFR^−/− BM cells or with a mixture of 1:10 CD45.1⁺ WT and CD45.2⁺ MCSFR^−/− fetal liver cells. Bar graphs show the % of CD45.2⁺ KO and CD45.1⁺ WT cells among blood cells (granulocytes or monocytes) used as a control and each SB LP DC subset. Error bars represent means +/− SD from 3 simultaneously analyzed experiments. (*) − 0.05>p>0.005, (**) − p<0.005 as compared to the chimerism of blood granulocytes or monocytes.