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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4052–4061. doi: 10.4049/jimmunol.181.6.4052

Figure 2.

Figure 2

Vessels within ILFs express MAdCAM-1 and stromal cells within ILFs cells express VCAM-1. To evaluate the expression of the ligands for α4β1 and α4β7, frozen sections of ILFs from BALB/c were stained with anti mouse MAdCAM-1 and VCAM-1 as described in material and methods. The expression of MAdCAM-1 localized to structures that had the appearance of vessels (panel A), which was confirmed by positive staining for CD31 (panels B and C: serial sections of a vessel at higher magnification stained with anti-MAdCAM-1 and CD31 respectively). These vascular structures displayed low or no PNAd expression (not shown). VCAM-1 expression was diffusely distributed in the ILF, and primarily restricted to CD45− (non-hematopoietic) stromal cells (panel D). Scale bar in panel A = 100µm.

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