Figure 4. Dorsal – ventral patterning defects in alien mutants.
Coronal sections of E12.5 wild-type (A,C,E,G,I,K,M) and aln (B,D,F,H,J,L,N) embryos showing expression patterns of indicated markers. The forebrain does retain telencephalic character in aln mutants (A,B). We note the presence of all markers analyzed but a decrease in dorsal gene expression (C–H: Wnt3a, Lhx5, Ngn2) and an increase in ventral markers Mash1 (I,J) and Dlx2 (K,L). The expression of Nkx2.1 (M,N) does not extend significantly beyond the wild-type domain.