Table 1.

Plasmids

Plasmid	Relevant characteristic(s)	Prototype plasmid	Drug resistance	Source and/or reference*
pYNEC106	ecoRVRMC	Unknown	none	This work, (27)
pYNEC107	ecoRVRMC	pBR322	Ap	This work, (27)
pYNEC111	ecoRVRMC	pBR322	Ap	This work
pYNEC112	ecoRVRMC	pUC19	Ap	This work
pYNEC113	ecoRVRMC	pBR322	Ap	This work
pYNEC114	ecoRVRMC	pHSG415	Ap, Cm	This work
pYNEC115	ecoRVR−MC	pUC19	Ap	This work
pYNEC116	ecoRVRMC−	pUC19	Ap	This work
pYNEC117	ecoRVR−MC	pBR322	Ap	This work
pYNEC118	ecoRVRMC−	pBR322	Ap	This work
pYNEC119	ecoRVR−MC	pHSG415	Ap, Cm	This work
pYNEC120	ecoRVRMC−	pHSG415	Ap, Cm	This work
pYNEC126	ecoRVC	plK153	Cm	This work
pYNEC130	ecoRVC−	plK153	Cm	This work
pPvuRM3.4	pvullRMC	pBR322	Ap	R. M. Blumenthal (Medical College of Ohio) (28)
pYNEC300	pvullRMC	pUC19	Ap	This work, (28)
pYNEC302	pvullR−MC	pUC19	Ap	This work, (19)
pYNEC303	pvullRMC−	pUC19	Ap	This work, (18)
pYNEC304	pvullR−MC−	pUC19	Ap	This work, (19)
pYNEC311	pvullC	plK153	Cm	This work, (19)
pYNEC323	pvullRMC	plK153	Cm	This work, (28)
pYNEC324	pvullR−MC	plK153	Cm	This work, (19)
pYNEC325	pvullRMC−	plK153	Cm	This work, (18)
pBamHIRM22	bamHlRMC	pTB53	Tc, Km	B. Kawakami (Toyobo) (29)
pYNEC401	bamHlC	plK158	Cm	This work
pYNEC403	bamHlRMC	pUC19	Ap	This work
pYNEC404	bamHlRMC−	pUC19	Ap	This work
pBR322			Ap, Tc	Laboratory stock
pHSG415	Temperature-sensitive for replication		Ap, Km, Cm	J. Kato (University of Tokyo) (30)
pKC31	λ phage replication origin	Bacteriophage λ	Km	(31)
plK153		pACYC184	Cm	K. Kusano (our laboratory)
plK158		pACYC184	Cm	K. Kusano (our laboratory)
pYNEC332	No BamHI site	pKC31	Km	This work, (31)
pYNEC333	No BamHI site	plK153	Cm	This work

^*Reference for DNA inserted to generate the listed plasmid.