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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Circulation. 2009 Jul 6;120(3):245–254. doi: 10.1161/CIRCULATIONAHA.109.868729

Table 2.

DPN-induced changes in protein expression identified by 2D CyDye DIGE studies.

Protein Name Protein ID Mass pI Arbitrary ratio of Cy3/Cy5
(i) Increased protein expression by DPN treatment
Aconitase Q99KI0 86151 7.87 1.65 ± 0.08
Myosin light chain 1 P09542 22521 5.03 1.52 ± 0.03
(ii) Decreased protein expression by DPN treatment
α-actin-2 P62737 42009 5.23 0.28 ± 0.06
Myosin regulatory light chain 2 P51667 18852 4.86 0.40 ± 0.07
Tropomyosin-2 P58774 32930 4.66 0.58 ± 0.04
ATP synthase subunit e Q06185 8230 9.34 0.60 ± 0.05

All proteins were positively identified (peptide count 2 or more, best ion score > 95%) in three individual 2D CyDye DIGE.