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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Cell Physiol. 2009 Nov;221(2):469–479. doi: 10.1002/jcp.21882

Fig. 6.

Fig. 6

Effect of O2 concentration on basal and Tat-induced transcription of HIV-1 LTR with deletion of NF-κB sequences and inactivation of Sp1 sites. 293T cells were transiently transfected with indicated HIV-1 LTR-Luciferase reporters without Tat (A) or with Tat (B). Lane 1, WT HIV-1 LTR (−105 to +77). Lane 2, HIV-1 LTR (−81 to +77) with NF-κB-deleted sites. Lane 3, HIV-1 LTR (−105 to +77) with Sp1 inactivated sites. The cells were cultured at 21% O2 or 3% O2 for 18 h. Then the cells were lysed and luciferase activity was measured.