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Expression of either Cx31 skin disease mutant also disrupts the ER marker BiP/GRP78 in NTERT cells but not SH-SY5Y cells. The ER marker BiP/GRP78 (E–H) is upregulated/disrupted in NTERT cells expressing (R42P)Cx31-EGFP (B) and (C86S)Cx31-EGFP (C) but not the (WT)Cx31-EGFP (A) or (66delD)Cx31-EGFP (D). In contrast, BiP/GRP78 (M–P) is unaffected by the expression of any Cx31-EGFP construct (I–L) in SH-SY5Y cells. The DAPI-stained nuclei are shown in blue.
