Table 1.

Experimental conditions of PCR: specific primers, annealing temperature (T), total number of amplification cycles (N) used for semi-quantitative PCR analysis of gene expression and corresponding size of the amplified fragments (S)

Gene (accession number)	Primer sequence (5′ → 3′)		T (°C)	N	S (bp)
LuEF1α	Sense	TTGGATACAACCCCGACAAAA	60	31	100
	Anti-sense	GGGCCCTTGTACCAGTCAAG
Lupmet	Sense	GGRCCATCRAARCACCARGC	55	30	158
	Anti-sense	AAGATGARGTCGACKGTSCC
Lupme1 (AF355056)	Sense	CATCAACCCCAACTTCCTCCTC	55	35	223
	Anti-sense	CCCACCACCGCAGTTTTAATATC
Lupme3 (AF188895)	Sense	ACCGGTCCATGTATCGC	55	32	178
	Anti-sense	TGACGTGCTCATTGTCTC
Lupme5 (AF355057)	Sense	CGCCGCCGTTTTATCC	53	35	176
	Anti-sense	CGCACAGCTCGATGCA
Flxper1 (L07554)	Sense	TACTTCACCAATCTCCAGACC	58	29	101
	Anti-sense	GCGAACCTGTTGACGAGCTC
Flxper2 (L24120)	Sense	CCACTCCGACCAAGTCCTGTT	58	32	133
	Anti-sense	CAGTGAGGGGCTTGATGTCT
Flxper3 (U59284)	Sense	CGACAACAAGTATTATGTTGACTTG	60	30	185
	Anti-sense	ACCGGTCAACACGCTTATCTGC
Flxper4 (AF049881)	Sense	TACCAGGATCTGGTGGCGAG	58	35	98
	Anti-sense	TTGTTGCTGTAAGTCCTCACC

A theoretical total Lupmet was obtained using degenerate primers designated as the most common sequences within the three Lupme genes.