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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5702–5710. doi: 10.4049/jimmunol.181.8.5702

FIGURE 4.

FIGURE 4

Knockdown of epithelial PAR-1 and -2 prevents the fall in TER induced by PMN contact. Inverted SK-CO15 monolayers cultured on 0.4-μm pore size filters were transfected with siRNA targeting cyclophilin B (control siRNA), PAR-1, PAR-2, or PAR-1 and -2. Semiquantitative RT-PCR analysis revealed decreased PAR-1 and -2 mRNA after transfection with PAR-1 and -2 siRNA, respectively. B, After epithelial siRNA pretreatment, PMNs were added to the basolateral aspect (upper compartment) of the monolayers cultured on 0.4-μm pore size filters while fMLP was added to the lower compartment. TER was measured after 3 h and is expressed as the percentage of baseline TER prior to addition of PMNs or vehicle. Values are means ± SEM, n = 3 per group; *, p < 0.05 compared with untransfected epithelial monolayers incubated with an fMLP gradient; †, p < 0.05 compared with PMNs added to the basolateral aspect of epithelial monolayers transfected with cyclophilin B siRNA or to untransfected epithelial monolayers. Data were generated from three independent experiments done in duplicate.