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. 2009 Oct 6;101(9):1585–1595. doi: 10.1038/sj.bjc.6605308

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Copyright © 2009 Cancer Research UK

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Caspase-3 activity and cell viability after treatment with zVAD and curcumin. (A) All four cell lines were treated with 25 μM of curcumin for 24 h. Active caspase-3 activity was estimated by western blotting using equal amounts (40 μg) of whole-cell lysates. β-Actin was used as a loading control. The western blot shown is representative of three independent experiments. (B) Cell viability estimated by MTT assay in all four oesophageal cancer cell lines after treatment with curcumin and pan-caspase inhibitor zVAD-fmk for 24 h. Data was expressed as the percentage of the control. ^*P<0.05 and ^**P<0.005 compared with untreated cells (paired Student's t-test). Bars=s.d.