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. 2009 Oct 6;101(9):1585–1595. doi: 10.1038/sj.bjc.6605308

Figure 6.

Figure 6

Poly-ubiquitination and cyclin B1 expression in oesophageal cancer cell lines after treatment with increasing concentrations of curcumin for 24 h. (A) Effects of curcumin treatment on poly-ubiquitination. Equal amounts (30 μg) of whole-cell lysates were separated using SDS–PAGE and protein poly-ubiquitination assessed by western blotting with an anti-ubiquitin antibody. Accumulation of high-molecular-weight ubiquitin complex is evident after curcumin treatment. (B) Cyclin B1 expression in oesophageal squamous and adenocarcinoma cell lines. Equal amounts (30 μg) of whole-cell lysates were separated using SDS–PAGE and protein expression assessed by western blotting with an anti-cyclin B1 antibody. β-Actin was used as a loading control. The western blot shown is representative of three independent experiments.