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. 2009 Sep 22;101(9):1606–1612. doi: 10.1038/sj.bjc.6605335

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Copyright © 2009 Cancer Research UK

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Apoptosis induced by mutant p53 knockdown is independent of TAp63 or TAp73 function. (A) Knockdown of mutant p53 does not induce the p53 pro-apoptotic targets, PUMA and NOXA, in T47D. T47D cells were harvested at different time points after p53 shRNA infection and were analysed by immunoblotting with PUMA- and NOXA-specific antibodies. β-Tubulin served as loading control. (B) Specific and effective knockdown of endogenous TAp63 and TAp73 in MDA-MB-468 cells. Pools of cells that stably express a TAp73-targeted shRNA (TAp73si) or TAp63-targeted shRNA (TAp63si) were generated through infection of the respective shRNAs, followed by a brief drug selection. The expression level of TAp63 and TAp73 in the stable cell line was evaluated by QRT-PCR. (C) Induction of apoptosis after mutant p53 knockdown is independent of TAp73 and TAp63 function in T47D and MDA-MB-468 cells. TAp73- or TAp63-ablated cells were infected with p53-directed lentiviral shRNAs or controls, and lysates were harvested at 72 h for immunoblot. (D) Quantitation of apoptosis by annexin V/PI staining of cells 96 h after mutant p53 knockdown. Error bars represent s.d. for three independent experiments.