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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Feb;62(2):612–619. doi: 10.1073/pnas.62.2.612

A FLUORESCENCE PROBE OF ENERGY-DEPENDENT STRUCTURE CHANGES IN FRAGMENTED MEMBRANES*

A Azzi 1, B Chance 1, G K Radda 1,†, C P Lee 1
PMCID: PMC277854  PMID: 4307717

Abstract

The reaction of the fluorochrome, 8-anilino-1-naphthalene-sulfonic acid (ANS), with fragmented membranes from beef heart mitochondria has been studied. ANS fluorescence is found to be enhanced 25-fold on binding to the membrane fragments in the absence of energy conservation, and this enhancement is increased to 35-fold in the membrane energized by substrate plux oxygen. The fluorescence of bound ANS depends upon the energy state of the membrane fragments, as indicated by the effects of ATP, substrates of the respiratory chain, oligomycin, and uncouplers. It is concluded that the changes of ANS fluorescence indicate structural changes of the mitochondrial membrane associated with energy conservation. The time course of energization is readily followed by ANS, and has a half-time of two seconds at 26°.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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