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. 2009 Nov 30;4(11):e8050. doi: 10.1371/journal.pone.0008050

Figure 4. Peptide analysis using LTQ-FT mass spectrometer.

Figure 4

(A) MS and MS/MS of HPLC fraction 25 that was recognized by MCAs 2077 and F11. The inset is the fragmentation (MS/MS) spectrum of the dominant peptide at m/z 781 [MH2 (2+)]. These experimental MS/MS fragment ions match to theoretical fragment ions produced by peptide ALEDDVIDGYPVKK, corresponding to amino acids 377–390 of human aromatase. (B) MS and MS/MS of HPLC fraction 46 that was recognized by MCA 677. The inset is the fragmentation (MS/MS) spectrum of the dominant peptide at m/z 729 [MH2 (2+)]. These experimental MS/MS fragment ions match to theoretical fragment ions produced by peptide DLKDAIEVLIAEK, corresponding to amino acids 250–262 of human aromatase.