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. 2009 Nov 30;4(11):e8050. doi: 10.1371/journal.pone.0008050

Figure 6. Validation of predicted function of residue lysine-108.

Figure 6

(A) Amino acid sequences alignment of the B' helix of the aromatase family. (B) Aromatase activity assay of WT aromatase and the K108Q mutant in CHO cells. (C) Western blot analysis on whole-cell lysates from CHO cells control or transfected with WT or K108Q aromatase using aromatase polyclonal antibody. (D) Substrate binding spectrum of the K108Q aromatase in the presence of androstenedione.