Figure 7. Lineweaver–Burk plots of aromatase kinetic analysis.

(A) and (C). Kinetic analysis of recombinant aromatase or the K108Q mutant with substrate androstenedione (from 10 nM to 500 nM) in the presence of reductase (from 2 nM to 20 nM). (B) and (D). Kinetic analysis of recombinant aromatase or the K108Q mutant with substrate reductase (from 1 nM to 20 nM) in the presence of androstenedione (from 10 nM to 500 nM). Aromatase activity assay were performed by the tritiated water release method using [1β-³H(N)]-androstenedione with 50 nM pure aromatase protein.