Table 1.
Tenascin-C, binding partners and cell responses
| TN-C domains | Ligand | Effect | Reference |
|---|---|---|---|
| TN-EGF-repeats | Counteradhesive for fibroblasts, neurons, glial cells | Spring et al. 1989; Prieto et al. 1992 | |
| Neuronal outgrowth, neurite repulsion | Fischer et al. 1997; Gotz et al. 1997 | ||
| Rounding of NIH3T3 cells, suppression of growth; reduced intracelular pH | Crossin 1991 | ||
| TNegf1,2; TNegf11-14 | EGFR | EGFR phosphorylation | Swindle et al. 2001 |
| PLCγ activation; fibroblast migration | Iyers et al. 2007 | ||
| TNfnIII1-3 | Cerebellar neuron attachment | Gotz et al. 1997 | |
| Suppression of milk protein synthesis in mammary epithelium, tissue involution | Jones et al. 1995 | ||
| TNfnIII1-5 | Fibronectin | T lymphocyte inhibition of β1 integrin adhesion | Hauzenberger et al. 1999 |
| TNfnIII2-6 | Integrins | Cell attachment | Prieto et al. 1992 |
| TNfnIII3 | αvβ3, α2β1 | EC elongation | Sriramarao and Bourdon 1993 |
| αvβ3, αvβ6 | Glioma spreading | Prieto et al. 1993 | |
| αvβ3, α9β1, αvβ6 | Colon carcinoma spreading | Yokosaki et al. 1996 | |
| αvβ1 | Neuron attachment, inhibition of growth | Probstmeier and Pesheva 1999 | |
| α8β1 | Smooth muscle cell, astrocyte adhesion | Schnapp et al. 1995 | |
| α8β1 | K562 cell adhesion | Denda et al. 1998 | |
| TNfnIII3-5 | Perlecan | CHO cells, incorporation of TNC into fibronectin-rich matrix | Chung and Erickson 1997 |
| TNfnIII3,5,6 | αvβ1 | Glioma process extension | Giese et al. 1996; Phillips et al. 1998 |
| TNfnIIIA-D | Attachment of uterine epithelia, reduced adhesion and implantation | Julian et al. 1994 Matsuda et al. 1999 | |
| Annexin II | Corneal epithelia proliferation, migration, wound healing. Endothelia, loss of focal adhesions, increase in mitogenesis and migration | Chung and Erickson 1994; 1996 | |
| TNfnIIIA1A2 | Fibronectin | Inhibition of lymphocyte activation | Puente Navazo et al. 2001; Parekh et al., 2005 |
| CD3 | Inhibition of tumor infiltrating lymphocytes, inhibition of proliferation, blocking secretion of cytokines | ||
| TNfnIIIA2 | HS, Syndecan-4 | Cell spreading | Saito et al. 2007 |
| Binding of YTITIRG to the HS side chain activates syndecan-4 and clustering with β1 integrins | |||
| TNfnIIIA3 | MMP-2, MMP-3 | Fetal membranes, tissue remodeling | Bell et al. 1999 |
| TNfnIIID | α7β1 | Neurite outgrowth | Mercado et al. 2004 |
| contactin | Activation of PKC and calmodulin kinase | Michele and Faissner 2009 | |
| TNfnIII6-8 | Fibronectin | Incorporation of TNC into matrix | Chiquet-Ehrismann et al. 1991;Chung et al. 1995 |
| TNfnIII7-8 | Modulation of FN-dependent induction of c-fos, collagenase, stromelysin and gelatinase expression in synovial fibroblasts | Tremble et al. 1994 | |
| TN-FBG | Integrin αvβ3 | Attachment of integrin transfected CHO cells | Yokoyama et al. 2000 |
| bFGF-induced sprouting, elongation of aortic ECs | Schenk et al. 1999 | ||
| HSPG-dependent induction of bone marrow mononuclear cell, supports growth in integrin independent manner | Seiffert et al. 1998 | ||
| Phosphacan/RPTPζ/β | 12nM kd binding, inhibits neuronal adhesion | Aukhil et al. 1993 | |
| TLR4 | Activation of TLR4 and induction of inflammatory cytokines | Midwood et al. 2009 |
Summary of mapped interaction sites within tenascin-C and transmembrane receptors, MMPs and ECM molecules. The described cellular responses and corresponding signaling pathways are listed. Full references are found at the end of the article. bFGF, basic fibroblast growth factor, FN, fibronectin, HS, heparan sulfate, HSPG, heparan sulfated proteoglycan, PKC, protein kinase C, MMP, metalloprotease