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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6265–6274. doi: 10.4049/jimmunol.181.9.6265

FIGURE 2.

FIGURE 2

Ikaros can repress Notch target gene expression in the presence of ICN. A, Twenty-four hours postinfection, JE131, DO11, and Tu5 cells successfully infected with MSCV IRES H-2Kk (Ik-) or MSCV IRES Ik-1 H-2Kk (Ik-1) retroviruses were purified using H-2Kk as a marker of successful transduction. cDNA was prepared and analyzed by qRT-PCR for expression of Hes1, Deltex1, and pTa. Y-axis shows target gene expression normalized to expression of the housekeeping gene, HPRT, in each sample. Values were determined as described in the legend for Fig. 1. Experiments were performed at least three times in duplicate from two independent RNA samples. p values were calculated using paired student T tests (*, p ≤ 0.05; **, p ≤ 0.03). Error bars represent SEM. B, Whole cell extracts from successfully transduced JE131 cell populations prepared as described above were subjected to Western blot analysis using Abs targeted against ICN. Blots were also probed with anti-actin Abs as a loading control.