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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6265–6274. doi: 10.4049/jimmunol.181.9.6265

FIGURE 5.

FIGURE 5

Ikaros null thymic progenitors require interaction with Notch ligand to differentiate and proliferate. A, Equal numbers of DN thymic progenitors from wild-type (Ik+/+) and Ikaros null (Ik−/−) mice were cocultured with the OP9 DL1 (62) or OP9 GFP (bottom) stromal cell line. Flow cytometric plots of cells stained with fluorochrome-conjugated Abs against CD4 and CD8 indicate the differentiation status of cells in each culture over time (days 0 to 4). Experiment was performed four times. Representative plots are displayed. B, DN thymic progenitors from Ik+/+ and Ik−/− mice were cultured on OP9 DL1 stroma in the presence or absence of γ-secretase inhibitor XII (GSI) over a 4-day period. OP9 GFP cultures serve as a control for differentiation in the absence of exogenous Notch signals. Experiment was performed twice. Representative plots are displayed. C, Proliferation curves are from the cultures whose differentiation status is depicted in A. Experiment was performed three times. Representative data are shown.