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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6265–6274. doi: 10.4049/jimmunol.181.9.6265

FIGURE 6.

FIGURE 6

Deregulation of Notch target genes is observed in Ikaros null single positive thymocytes. qRT-PCR analysis was performed using cDNA prepared from sorted CD4+ and CD8+ single positive (19) thymocyte subsets from Ikaros null (Ik−/−) and wild-type (Ik+/+) mice. Expression levels of the indicated Notch target genes were assessed. Y-axis shows target gene expression normalized to expression of the housekeeping gene, HPRT, in each sample. Values were determined as described in the legend for Fig. 1. Experiments were performed at least three times in duplicate from two independent RNA samples. p values were calculated using paired Student t tests (*, p ≤ 0.03; **, p ≤ 0.01). Error bars represent SEM.