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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6265–6274. doi: 10.4049/jimmunol.181.9.6265

FIGURE 7.

FIGURE 7

Ikaros null bone marrow progenitors do not display derepressed Notch target gene expression. A, qRT-PCR was performed using cDNA prepared from lineage depleted bone marrow from Ikaros null (Ik−/−) and wild-type (Ik+/+) mice. Expression levels of the indicated Notch target genes were assessed. Y-axis shows target gene expression normalized to expression of the housekeeping gene, HPRT, in each sample. Values were determined as described in the legend for Fig. 1. Experiments were performed at least three times in duplicate from two independent RNA samples. p values were calculated using paired Student t tests (*, p ≤ 0.02). Error bars represent SEM. B, Whole bone marrow was isolated from Ikaros null (Ik−/−) and wild-type (Ik+/+) mice. Flow cytometric plots of cells stained with fluorochrome-conjugated Abs against CD4 and CD8 are shown. Experiment was performed twice. Representative plots are shown.