Table 1.
Phosphorylated peptides identified from membrane fractions shaved by protease.
| Protein name | GO annotation | ATG number | TMD | Label-free ratio (spectrum) | Label-free ratio (chromatogram) | D/H ratio (spectrum) | D/H ratio (chromatogram) | Phosphopeptide sequence and phosphorylation site |
|---|---|---|---|---|---|---|---|---|
| Membrane protein | ||||||||
| ATPase 1, plasma membrane-type | ATPase | At2g18960 | 10 | 5.62 + 3.01 | 6.92 + 3.83 | GLDIDTAGHHYpTV | ||
| Sugar transport protein 1 | Transporter | At1g11260 | 4.36 | 7.95 | GVDDVpSQEF DDLVAASK |
|||
| Patellin-2 | Transporter | At1g22530 | 2.91 + 0.24 | 8.37 + 4.03 | EILQSEpSFKEEGY LASELQEAEK |
|||
| Aquaporin PIP2;1, PIP2;2; PIP2;3 | Water channel | At3g53420 | 6 | 0.33 + 0.10 | 0.35 + 0.13 | 0.46 | 0.49 | SLGpSFRpSAANV |
| At2g37170 | ||||||||
| At2g37180 | ||||||||
| Probable aquaporin PIP2;4 | Water channel | At5g60660 | 6 | 1.22 + 0.05 | 1.24 + 0.55 | ALGSFGpSFGSFR | ||
| Probable aquaporin PIP2;4 | Water channel | At5g60660 | 6 | 1.22 + 0.05 | 1.24 + 0.55 | ALGSFGSFGpSFR | ||
| Probable aquaporin PIP2;4 | Water channel | At5g60660 | 6 | 3.37 + 1.22 | 2.38 + 0.15 | 0.52 | 0.61 | ALGSFGpSFGpSFR |
| Probable inactive receptor kinase | kinase | At3g02880 | 2 | 2.07 + 0.31 | 3.99 + 1.94 | 1.68 | 1.61 | LIEEVSHSS GSPNPVpSD |
| Probable purine permease 18 | Transporter | At1g57990 | 10 | 7.84 + 0.47 | 5.31 + 1.03 | QTTAEGSANP EPDQILpSPR |
||
| Non-membrane protein | ||||||||
| Mitogen-activated protein kinase kinase 2 | Kinase | At4g29810 | 1.08 | 0.61 | IISQLEPEVLpSPIKPA DDQLSLSDLDMVK |
|||
| Uncharacterized TPR repeat-containing protein | Unknown | At1g05150 | 2.82 | 4.28 | DNDVPVpSY SGSGGPTK |
|||
| FAM10 family protein | Binding | At4g22670 | 0.45 + 0.37 | 0.63 + 0.36 | 0.54 | 0.56 | VEEEEEEDEIVEpSDVEL EGDTVEPDNDPPQK |
|
| FAM10 family protein | Binding | At4g22670 | 0.20 | 0.17 | SFVVEEpSDDD MDETEEVKPK |
|||
| 60S ribosomal protein L13-1 | Translation | At3g49010 | 1.61 + 0.53 | 1.28 + 0.41 | AGDSpTPEELANA TQVQGDYLPIVR |
|||
| 60S acidic ribosomal protein P1-1, P1-2, P1-3 | Translation | At1g01100 | 0.14 + 0.02 | 0.24 + 0.02 | 0.29 | 0.11 | KKDEPAEEpS DGDLGFGLFD |
|
| At5g47700 | ||||||||
| At4g00810 | ||||||||
| 60S acidic ribosomal protein P2-1, P2-2, P2-4 | Translation | At3g44590 | 0.26 + 0.05 | 0.24 + 0.02 | KEEKEEpSD DDMGFSLFE |
|||
| At2g27710 | ||||||||
| At2g27720 | ||||||||
| 60S acidic ribosomal protein P3-1, P3-2 | Translation | At5g57290 | 0.44 + 0.14 | 0.68 + 0.27 | 0.29 | 0.37 | KEEpSEEEE GDFGFDLFG |
|
| At4g25890 | ||||||||
| 60S acidic ribosomal protein P0-3 | Translation | At3g11250 | 0.12 + 0.04 | 0.13 + 0.05 | 0.21 | 0.27 | VEEKKEEpSDEED YEGGFGLFDEE |
1. AGI code: the accession number refers to Arabidopsis Gene Idenfier code.
2. TMD: predicted transmembrane domain number.
3. Label-free ratio represents fold difference of peptide level between 200 mM-sample versus control.
4. FDR is calculated 0 based on decoy database search.
5. D/H ratio represents fold difference of peptide level between 200 mM-sample versus control. D: 200 mM; H: control.
6. Each peptide was corroborated by Scaffold using 95% confidence interval as the cut-off.