Table 2.
Selected list of genes associated with 1,25D resistance identified by expression array analysisa
| Gene symbol |
Accession Number [GenBank:] |
Gene description | Chromosome location |
Expression array (log2 ratio) |
Q-PCR |
|---|---|---|---|---|---|
| Top 5 down-regulated | |||||
| FAM14A | NM_032036 | Family with sequence similarity 14, member A | 14q32.13 | -2.03 | -6.79 |
| CYP24A1 | NM_000782 | Cytochrome P450 (Vitamin D 24-hydroxylase) | 20q13 | -1.20 | - |
| PHLDA1 | NM_007350 | Pleckstrin homology-like domain, family A, member 1 | 12q15 | -1.11 | -3.69 |
| ZFP276 | NM_152287 | Zinc finger protein 276 | 16q24.3 | -1.02 | - |
| - | BF244871 | cDNA clone IMAGE:4083158 | 11 | -0.98 | - |
| Top 5 up-regulated | |||||
| SLITRK6 | NM_032229 | SLIT and NTRK-like family, member 6 | 13q31.1 | 0.98 | - |
| BMP7 | AK094784 | Bone morphogenetic protein 7 | 20q13 | 0.96 | - |
| - | BG115630 | cDNA clone IMAGE:4417140 | 4 | 0.95 | - |
| DDIT4 | NM_019058 | DNA-damage-inducible transcript 4 | 10pter-q26.12 | 0.91 | 2.64 |
| CYFIP2 | NM_001037332 | Cytoplasmic FMR1 interacting protein 2 | 5q33.3 | 0.89 | 1.98 |
| Additionalb | |||||
| FOXC1 | NM_001453 | Forkhead box C1 | 6p25 | -0.80 | -2.05 |
| CRABP2 | NM_001878 | Cellular retinoic acid binding protein 2 | 1q21.3 | -0.66 | -2.69 |
| ST6GALNAC5 | NM_030965 | GalNAc alpha-2,6-sialyltransferase V | 1p31.1 | -0.10 | -0.39 |
| NUAK1 | NM_014840 | NUAK family, SNF1-like kinase, 1 | 12q23.3 | 0.75 | 2.66 |
| VDR | NM_000376 | Vitamin D receptor | 12q13.11 | - | 0.13 |
-. Not available
a. Genes were identified considering FDR<0.05 as significant, with the exception of VDR due to flagging (see text for explanation)
b. Additional genes selected for Q-PCR quantification