Fig. 10.

Ethidium-stained gel of RT-PCR results for a patient with suspected synovial sarcoma. Total RNA was extracted from formalin-fixed, paraffin-embedded tissue and subjected to RT-PCR. M, A DNA molecular weight marker (100-bp ladder); lanes 1 and 2, patient sample run in duplicate is negative for a SS18/SSX1 fusion transcript; lane 3, SS18/SSX1 fusion transcript 110-bp positive control; lanes 4 and 8, negative no template control; lanes 5 and 6, patient sample run in duplicate is positive for a 110-bp SS18/SSX2 fusion transcript; lane 7, SS18/SSX2 fusion transcript 200-bp variant positive control