Fig. 7.

Only one allele of Stan2 is expressed in progeny with colored skin. Primers Stan2-F4 and Stan2-ClaI-R1 were used to amplify cDNA prepared from red- and purple-skinned progeny of the cross between W5281.2 and 07506-01. Amplification products were then digested with Alu I and separated on a 2% agarose gel to determine which W5281.2 and 07506-01 alleles are transcribed. a Predicted fragment sizes. Complementary DNA of Stan2 ⁷⁷⁷ was predicted to give restriction fragments of 132, 119, 100, 52, and 44 bp. Based on genomic DNA sequence, the other allele of W5281.2 and one allele of 07506-01 were predicted to give fragments of 219, 132, 52, and 44 bp, while the remaining allele of 07506-01 was predicted to give fragments of 219 and 186 bp. b Actual fragment sizes observed. Panel shows an ethidium-bromide-stained gel and demonstrates that only Stan2 ⁷⁷⁷ was expressed