Fig. 8.

Transformation of the cultivars Désirée and Bintje, and two progeny from a cross between W5281.2 and 07506-01, with Stan2. The open reading frame of the R2R3 MYB domain allele Stan2 ⁷⁷⁷ was placed into the vector pPS1 under the control of a doubled 35S promoter and introduced via Agrobacterium-mediated transformation. Tubers were harvested from mature plants and photographed several days later. a Foliage, tubers, and tuber flesh of cultivar Désirée, as well as corresponding tissues from a Désirée plant transformed with Stan2 ⁷⁷⁷. Top right thin layer chromatography of anthocyanin pigments isolated from tuber skin of untransformed Désirée, Désirée transformed with Stan2 ⁷⁷⁷, and Désirée transformed with a functional allele of f3′5′h (Jung et al. 2005). Pigment samples were applied near the bottom of the TLC plate shown and developed with a solvent comprised butanol/2 N HCl. The orange color and faster migration of the sample prepared from Désirée and Désirée transformed with Stan2 ⁷⁷⁷ is characteristic of derivatives of pelargonidin, while the purple color and slower migration in the remaining two lanes is characteristic of derivatives of petunidin (Dodds and Long 1955). b Tuber and tuber flesh of Bintje, as well as corresponding tissues from Bintje transformed with Stan2 ⁷⁷⁷. c Tuber and tuber flesh of a diploid clone with genotype dd Rr pp, as well as corresponding tissues from the same clone transformed with Stan2 ⁷⁷⁷. d Tuber and tuber flesh of a diploid clone with genotype dd Rr Pp, as well as corresponding tissues from the same clone transformed with Stan2 ⁷⁷⁷