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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: Eur J Oral Sci. 2009 Oct;117(5):518–527. doi: 10.1111/j.1600-0722.2009.00651.x

Fig. 4.

Fig. 4

Interaction of migfilin with kindlin-1. A, GST-kindlin-1 specifically binds to migfilin in human 293 cells. GST-kindlin-1-bound glutathione-Sepharose or control beads were incubated with cell lysates and subjected to Western blotting using anti-migfilin antibody. Lane 1, cell lysate; lane 2, cell lysate incubated with GST-Sepaharose beads; lane 3, cell lysate incubated with GST-kindlin-1 beads. B, Immunolocalization of kindlin-1 (green) at the end of the actin filaments (red) in keratinocytes; C, immunolocalization of migfilin (red) at the end of actin filaments in keratinocytes; D–F, double immunolocalization of kindlin-1 (D, green) and migfilin (E, red) in same focal adhesions (F, orange). Arrows point to the focal adhesions.