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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: Eur J Oral Sci. 2009 Oct;117(5):518–527. doi: 10.1111/j.1600-0722.2009.00651.x

Fig. 5.

Fig. 5

Effect of kindlin-1 knockdown on migfilin and paxillin containing focal adhesions. A, expression of kindlin-1 mRNA after 0–3 days following kindlin-1 siRNA transfection relative to transfection with the control siRNA; B, expression of kindlin-1 protein 1–3 days after transfection with the kindlin-1 siRNA (K1) or irrelevant control siRNA (IR). β-actin was used as a loading control; C, Immunolocalization of migfilin and paxillin containing focal adhesions after transfection with kindlin-1 or IR siRNA; D, average number of migfilin containing focal adhesions per cell after siRNA transfections; E–F, distribution of migfilin (E) and paxillin (F) containing focal adhesions according to the size (0–2 µm, 2–5 µm, 5–100 µm) after siRNA transfection. All histograms show the mean +/− SEM of three parallel experiments (* p<0.05, **p<0.01).