Fig. 1.

Phenotypic and gene expression analysis of Shh conditional knockout GTs. (A-E) Scanning electron microscopy (SEM) analyses of E15.5 male genital tubercles (GTs) of control (A) and Shh-cKO (B-E) mice. The timing of Tamoxifen (Tm) treatment is indicated. Proximal hypospadias is indicated by arrows in B-E. (A′,C′-E′). Hematoxylin and Eosin (H&E)-stained cross-sections of wild-type (A′) and late Shh-cKO (C′-E′) GTs. Urethral epithelium and mesenchymal condensations are indicated by arrowheads and arrows, respectively. (F-K′) Whole-mount in situ analysis on E12.5 GTs from Shh-cKOs (F′-K′) and littermate controls (F-K). All embryos were exposed to Tm at E10.5.