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Multiple regions, but not the small GTPase activity, of Arl13b are required for its targeting to the cilium. (A) Images show side views of the pronephric duct in embryos injected with mRNA encoding eGFP-tagged versions of Arl13b indicated above. eGFP signal is shown in green, while anti-acetylated tubulin (Ac-tub) is labeled in red. CC, coiled-coil domain. Scale bars: 10 μm. (B) Schematic of the structure of Arl13b. Numbers are amino acid coordinates.
