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. 2009 Dec 1;136(23):4055–4063. doi: 10.1242/dev.036624

Fig. 2.

Fig. 2.

Neuroepithelial cells display a dorsal identity. (A) At 1 month after differentiation in the absence of exogenous morphogens, the majority of cells were positive for both FOXG1 and PAX6, whereas almost no cells were NKX2-1+. (B) qPCR showed the high expression level of PAX6 and EMX1 mRNA, but low level of NKX2-1 and DLX2 mRNA by NE cells at day 17. (C) Microarray analysis indicated little change in SHH signaling components except a significant increase in GLI3. (D) There is an increase in Wnt proteins and frizzled (FZD) proteins after day 6 of differentiation. (E) WNT3A-conditioned medium upregulated, whereas DKK1 (100 ng/ml, from day 10 to day 17) decreased, GLI3 expression at the mRNA level as revealed by qPCR. (F) Addition of WNT3A-conditioned medium from day 10 to day 17 resulted in an increase in the expression of PAX6 and EMX1 mRNA. Data are presented as mean±s.d. *, P<0.05 versus the control group, n=4. Scale bar: 50 μm.