Fig. 5.

Differentiation of glutamatergic and GABAergic neurons from dorsal and ventral progenitors. (A,B) Comparison of the expression of TBR1, CTIP2 and ISLET1 between the neurons that were developed from dorsal (control group) and ventral (DKK1 plus SHH group) progenitors. (C-E) Dorsal progenitors differentiated into cells that were positive for TBR1 (C), CTIP2 (D) and VGLUT1 (E). (F-H) Ventral progenitors differentiated into neurons that expressed GAD (F), GABA (G) and DARPP32 (H). (I) Western blot analysis showing differential expression of TBR1, DARPP32 and GAD65/67 between cultures patterned for dorsal and ventral identities. Blue, Hoechst-stained nuclei. Scale bars: 50 μm.