Figure 1.

The distribution of PSD-95 and pan-AMPAr immunoreactivity in the dorsal horn of 1-week-old and adult rats, seen after antigen retrieval with pepsin. In each row, PSD-95 (red) is on the left, pan-AMPAr (pAMPAr, green) is in the middle, and a merged image is shown on the right. Note that the distribution of each type of staining is similar at the two ages, with the highest density in a region of the superficial dorsal horn that corresponds to lamina II. The myelin bundles (which appear as unstained areas in the medial part of the dorsal horn) are only present in the adult. Each image was obtained from a single optical section scanned with a confocal microscope. Scale bar, 100 μm.