Figure 2.

Phagocytosis stimulated by fAβ requires CD14, TLR4, and TLR2. A, BV2 microglia were pretreated with function blocking antibodies to CD14 (MY4), TLR4 (HTA125), or TLR2 (T2.5), before stimulation with fAβ. Fluorescent microspheres were then added for 30 min. Cells were stained with phalloidin for visualization of actin and DAPI. Magnification is 40×. B, Confocal images (100×) of BV2 microglia treated with fAβ-containing microspheres in both the x–y plane (left) and z-scan cut (right) through the cells. C, Primary microglia from C57BL/6 (WT), CD14^−/−, TLR4^−/−, or TLR2^−/− mice were stimulated with fAβ, followed by incubation with fluorescent microspheres. The fraction of cells containing microspheres was determined by counting three random fields (>100 cells) on an inverted microscope. The data shown are the mean ± SEM of three independent experiments performed in duplicate. ***p < 0.001 compared with Non-treated.