Figure 6.

Activation of the p38 MAP kinase by fAβ requires CD14, TLR4, and TLR2. A–D, Primary microglia from C57BL/6 (WT), CD14^−/−, TLR4^−/−, or TLR2^−/− mice were stimulated with fAβ (A), LPS (B, C), Pam3CSK4 (D), or immune IgG (B–D). Cell lysates were analyzed by Western blot analysis using an anti-phospho-p38 antibody. Blots were stripped and reprobed with an anti-p38 antibody as a protein loading control. Western blot band intensity of phosphorylated-p38 was normalized to p38 levels and expressed as relative density. Western blots are representative, and the densitometry data are the mean ± SEM from at least three independent experiments. **p < 0.01, ***p < 0.001 compared with Non-treated.