Figure 7.

Fibrillar Aβ-stimulated ROS production and phagocytosis requires the activity of p38. A, Primary microglia from C57BL/6 mice were pretreated with SB203580 before stimulation with fAβ. Reduction of NBT results in the formation of a dark blue formazan deposit viewable by a light microscope. Three random fields (>100 cells) were counted and the fraction of cells with blue formazan deposits was determined. The data shown are the mean ± SEM of three independent experiments performed in duplicate. ***p < 0.001 compared with Non-treated. B, BV2 microglia were pretreated with SB203580 before stimulation with fAβ. Fluorescent microspheres were added, following which the fraction of cells containing microspheres was determined by counting three random fields (>100 cells) on an inverted microscope. The data shown are the mean ± SEM of three independent experiments performed in duplicate. ***p < 0.001 compared with Non-treated.