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1D 15N-NMR spectra of the wild-type FMN domain in the oxidized state (Panel A) and the fully reduced state (Panel B). Panel C: 1D 15N-NMR spectrum of the wild-type FMN domain in the fully reduced state using the DEPT pulse sequence without broad-band decoupling. The coupling constants for the 15N3 and 15N5 atoms were observed to be 93.6 and 70.3 MHz, respectively, as indicated.
