. Author manuscript; available in PMC: 2010 Jun 16.

Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5131–5141. doi: 10.1021/bi900607q

Table 1.

¹⁵N Chemical Shift Values (ppm) for Free and Bound FMN in the Oxidized and Reduced States at pH 7.0, 300 °K

FMN in the Oxidized State							FMN in the Fully Reduced State

Atom	FMN^a	TARF^a,^b	WT FMN domain	-⁵³⁷Ala-Ala- Variant	P540A Variant	P541A Variant	FMNH₂^a	FMNH⁻^a	TARFH₂^a,^b	WT FMN domain	P540A Variant	P541A Variant
N1	190.8	199.9	189.0	188.8	189.5	189.0	128.0	182.6	116.7	186.4	186.8	187.1
N3	160.5	159.8	160.5	158.6	160.8	160.5	149.7	149.3	145.8	148.4	148.6	149.1
N5	334.7	344.3	321.5	332.8	322.1	320.0	58.0	57.7	60.4	47.1	47.4	45.5
N10	164.6	150.2	162.6	162.3	162.2	162.8	87.3	97.2	72.2	98.1	98.4	98.9

From Vervoort et al. (39).

Tetraacetylriboflavin in chloroform.